HPLC同时测定赤栀黄胶囊中栀子苷和芍药苷的含量

Determination of jasminoidin and paeoniflorin in Chizhihuang Capsules by HPLC

目的 :建立赤栀黄胶囊 (赤芍、栀子、大黄等 )高效液相质量控制标准。方法 :用Shim packC18柱 ,在 2 30nm处同时测定赤栀黄胶囊中指标成分栀子苷和芍药苷的含量。结果 :栀子苷线性范围为 0 .30～ 1.5 0 μg ,平均加样回收率99.5 8% ;RSD 1.5 7% (n =5 )。芍药苷线性范围为 0 .2 6～ 1.30 μg ,平均加样回收率 99.11% ;RSD 1.2 9% (n =5 )。 结论 :该实验方法简便易行 ,结果准确可靠 ,可用于该制剂的质量控制。

AIM: To establish a HPLC quality control for Chizhihuang Capsules(Radix Paeoniae Rubra, Fructus Gardeniae, Radix et Rhizoma Rhei, etc.). METHODS: Shim pack C 18 column was used, and jasminoidin and paeoniflorin were determined at the same time and detection wavelength at 230nm. RESULTS: The standard curve of jasminoidin was linear in the range of 0.30～1.50μg. The average recovery and RSD were 99.58 and 1.57% ( n =5), respectively. The standard curve of paeoniflorin was linear in the range of 0.26～ 1.30μg . The average recovery and RSD were 99.11 and 1.29% ( n =5), respectively. CONCLUSIONS: This method is a convenient, reliable and accurate for the quality control of Chizhihuang Capsules.