三七总皂苷注射液HPLC指纹图谱的比较分析

Study on HPLC fingerprinting of Radix Notoginseng Injections

目的 :用高效液相色谱法测定不同提取方法的三七总皂苷及注射液的指纹图谱。方法 :乙腈 水二元梯度洗脱。ZORBAXEclipseXDB C18柱 ,0～ 6min ,乙腈 2 0 %→ 30 % ;6～ 14min ,乙腈 30 %→ 4 0 % ;14 2 5min ,乙腈 4 0 %→ 30 % ;2 5～30min ,乙腈 30 %→ 2 0 %。流速 1.0mL·min-1,检测波长 2 0 3nm ;进样量 2 0 μL,柱温为 4 0℃。结果 :三七总皂苷注射液的指纹图谱中发现并确定了 7个共有峰 ,以三七皂苷R1为参比峰 ,其余 6峰的相对保留时间分别为 (1.14 6± 0 .1) % ,(2 .137± 0 .2 ) % ,(2 .2 0 4± 1.1) % ,(2 .35 2± 0 .9) % ,(2 .4 4 7± 0 .8) % ,(2 .733± 0 .7) % ,但相对峰面积差异显著。结论 :不同提取方法的三七总皂苷原料及注射液存在差异 ,HPLC指纹图谱可用于其质量控制。

AIM: To evaluate the quality of different Notoginseng Injections. METHODS: Different total saponins from Panax notoginseng and their injections were determined by HPLC fingerprint. The analysis was carried out on ZORBAX Eclipse XDB C 18 column, and gradient elution of acetonitrile and water were used acetonitrile from 20% to 30% in 0～6min, acetonitrile from 30% to 40% in 6～14min, acetonitrile from 40% to 30% in 14～25min, acetonitrile from 30% to 20%in 25～30min. The flow rate was 1.0mL·min -1 , the detection wavelength was at 203nm, 20μL sample was injected and the column temperature was set at 40℃. RESULTS: Seven common peaks were found and relative retention time and their RSD were 1.00 , (1.146 ±0.1)% , (2.137 ±0.2)% , (2.024 ±1.1)% , (2.352 ±0.9)% , (2.447 ±0.8)% and (2.733 ±0.7)% , respectively. Relative retention time of the marker peaks in different total saponins from Panax notoginseng and their injections were very similar, however, the relative areas of some marker peaks were obviously different. CONCLUSION: Quality of total saponins from Panax notoginseng extracted by different methods and their injections were different. HPLC fingerprint can be used to control their quality.