盆神经节海绵体内移植对双侧海绵体神经损伤后大鼠阴茎勃起功能的影响

Effects on erectile function of transplanted major pelvic ganglion into the corpus cavernosum of adult rats with bilateral cavernous nerve injury

目的 研究双侧海绵体神经离断后 ,自体盆神经节海绵体内移植对大鼠阴茎勃起功能的影响。方法 将 2 6只雄性SD大鼠 (3～ 6个月 ,30 0～ 4 0 0g 只 )随机分为 2组 :(1)实验组 (n =16 )行双侧海绵体神经离断及左盆神经节移植入左侧阴茎脚内 ;(2 )对照组 (n =10 )仅离断双侧海绵体神经。1个月及 3个月后阿朴吗啡皮下注射检测勃起功能 ,电刺激左阴茎脚移植区域或右侧盆神经节比较海绵体内压的变化 ,NADPH染色检测海绵体内一氧化氮合酶 (NOS)阳性神经纤维 ,透射电镜观察盆神经节移植后的存活情况。结果 两组大鼠 1个月及 3个月后注射阿朴吗啡后均无勃起反应 ;电刺激左阴茎脚盆神经节移植区域后亦不能导致勃起。 1个月后电刺激两组左阴茎脚后的海绵体内压变化分别为 (9 4 1± 3 2 0 )、(4 16± 2 5 8)cmH2 O(t=4 76 9,P <0 0 5 ) ,3个月后分别为 (13 6 7± 4 18)、(5 0 9± 2 74 )cmH2 O(t=9 18,P <0 0 5 )。两组大鼠左阴茎脚区域的NOS阳性神经纤维数目 1个月后分别为 (2 18 7± 2 4 5 )、(18 0± 3 7) (t=14 77,P <0 0 5 ) ,3个月后分别为 (183 2± 19 7)、(19 0± 3 8) (t =12 18,P <0 0 5 ) ;透射电镜证实了神经节移植后的存活。

Objective To investigate the effects on erectile function of transplanted major pelvic ganglion into the corpus cavernosum of adult male rats undergoing transection of bilateral cavernous nerves. Methods Twenty-six male Sprague-Dawley rats (3-4 month-old and 300-400 g/each) were divided into 2 groups: experimental group (transection of bilateral cavernous nerves and transplantation of left ganglion into left crus of penis, n =16) and control group (transection of bilateral cavernous nerves only, n =10).Erectile function was measured by injecting APO,and intracavernous pressure was measured 1 and 3 months afterwards by electrostimulating the right major pelvic ganglion or the left crus.Half animals in each group were sacrificed 1 and 3 months afterwards for detecting nNOS-containing nerve fibers of corpus cavernosum.Electron microscopy of the implanted area was performed to assess neuronal survival. Results Both of the two groups have no erectile response to APO injection.Electrostimulation on the right major pelvic ganglion and left crus failed to produce erection in experimental group.The mean pressure changes in the two groups,measured by stimulating the left crus,were (9.41±3.20) and (4.16±2.58) cmH 2O 1 month afterwards,and (13.67±4.18) and (5.09±2.74) cmH 2O 3 months afterwards,respectively ( P <0.05).An increased number of nNOS-containing nerve fibers in left crus was detected in experimental group 1 and 3 months later,compared with control one (218.7±24.5,18.0±3.7;183.2±19.7,19.0±3.8; P <0.05).Ultrastructure examination by transmission electron microscope confirmed the survival of the implanted ganglion. Conclusion Major pelvic ganglion can survive in the corpus cavernosum,and it has significant effects on the number of nNOS-containing nerve fibers and the alteration of intracavernous pressure.