α肾上腺素受体阻滞剂抑制前列腺癌细胞生长的研究

The growth inhibition effect of α-adrenoceptor antagonists on androgen-independent prostate cancer cell line

目的 比较两种α1 肾上腺素受体阻滞剂特拉唑嗪和阿夫唑嗪 ,α1 肾上腺素受体阻滞剂酚苄明对雄激素非依赖性前列腺癌细胞系PC 3和DU 14 5的作用。方法 将 5、10 μmol L浓度的特拉唑嗪、阿夫唑嗪与酚苄明与PC 3、DU 14 5细胞共培养 ,检测上述 3种药物对PC 3和DU 14 5细胞系细胞存活百分率、癌细胞集落形成能力及细胞周期的影响。结果 特拉唑嗪能将雄激素非依赖性前列腺癌细胞PC 3和DU 14 5阻滞在G1 期 ,从而抑制雄激素非依赖性前列腺癌细胞系PC 3和DU 14 5的生长及癌细胞集落形成的能力。阿夫唑嗪和酚苄明对PC 3和DU14 5的生长及癌细胞集落形成能力无明显影响。结论 特拉唑嗪能通过诱导雄激素非依赖性前列腺癌细胞G1 期阻滞来抑制其生长 ,考虑此药可用于治疗雄激素非依赖性前列腺癌。

Objective The aim of the present study is to compare the effects of two α1-adrenoceptor antagonist terazosin and alfuzosin together with one α-adrenoceptor antagonist phenoxybenzamine on androgen-independent prostate cancer cell lines PC-3 and DU145. Methods Two androgen- independent cell lines,PC-3 and DU145,were used to determine the cell viability,colony-forming ability as well as cell cycle characteristics after exposure to these three drugs. Results This study showed that terazosin inhibited not only prostate cancer cell growth but also colony-forming ability,which is the main target of clinical treatment.On the other hand,alfuzsion and phenoxybenzamine have no effect on cell viability and colony forming ability of PC-3 and DU145.In addition,the terazosin inhibits cell growth through G 1 phase cell cycle arrest. Conclusion This study provided the evidence that α1-adrenoceptor antagonist terazosin may have a therapeutic potential in the treatment of advanced androgen-independent prostate cancer.