摘要
运用RAPD技术对网翅蝗科Arcypteridae 4种蝗虫 2 4个个体的基因组DNA进行了多态性分析。在试用的2 4条随机引物中 ,筛选出 11条引物用于 4种蝗虫的随机引物扩增 ,共得到 12 8条清晰稳定的扩增片段 ,每条引物的扩增片段数为 10～ 13条 ,片段长度在 10 0～ 2 0 0 0bp之间。根据扩增出的RAPD图谱 ,用UPGMA和NJ法对Nei′s遗传距离作聚类分析 ,构建分子系统树。聚类结果显示 :同属的物种首先聚在一起 ;网翅蝗科 4种蝗虫分为两支 ,竹蝗属CeracrisWalker的贺氏竹蝗CeracrishoffmanniUvrov、黑翅竹蝗Ceracrisfasciatafsaciata (Br. W .)优先聚为一支 ,隆额网翅蝗ArcypteracoreanaShiraki和宽翅曲背蝗Pararcypteramicropterameridionalis (Ikonn)聚为另一支。基于RAPD图谱的分子系统树所展示的物种间亲缘关系与传统的形态分类结果基本一致 ,此结果表明RAPD在科下不同属间亲缘关系的研究方面具有一定的可行性。
Random Amplified Polymorphic DNA (RAPD)markers were applied to analyze genetic relationships among four species of Arcypteridae:Ceracris hoffmanni Uvrov,C. fasciata fsaciata (Br.-W.), Arcyptera coreana Shiraki and Pararcyptera microptera meridionalis(Ikonn.). Genomic DNA of twenty-four individuals was respectively amplified with eleven oligonucleotide (10*!mer) primers which were previously selected from twenty-four random primers (10*!bp), and a total of 128 clear and reproducible RAPD bands were observed. The amplified DNA fragments of individual primer were 10-13, and their molecular size was 100-2*!000*!bp. The dendrogram based on Nei′s genetic distance of RAPD markers was constructed using UPGMA and Neighbor-Joining. The cluster analysis showed that the species of each genus first clustered respectively and 4 species of Acridoidea were divided into two branches. One is C.hoffmanni Uvrov and C.fasciata fasciata (Br.-W.) cluster, and the other is A. coreana Shiraki and P. microptera meridionalis(Ikonn.) cluster. Whose conclusion coincided with the results of morphology, and this implied that RAPD seemed suitable for analysis of genetic relationships among genera.
出处
《动物分类学报》
CSCD
北大核心
2004年第2期212-217,共6页
Acta Zootaxonomica Sinica
基金
国家自然科学基金 (30 0 70 1 1 2
30 1 70 61 2 )
山西省自然科学基金资助项目 (991 0 96)
