铜离子对维生素C灭活噬菌体的增效作用

COPPER IONS AS SYNERGIST IN INACTIVATION OF BACTBRIOPHAGES BY ASCORBIC ACID

本实验观察了铜离子协同维生素C灭活噬菌体的效果。在灭活过程中, 维生素C起主要作用,铜离子起增效作用。单独维生素C灭活噬菌体的速度慢,铜离子与维生素C协同作用能快速灭活RNA噬菌体和DNA噬菌体。维生素C与铜的分子比为1:1～100:1灭活效果较好,铜的比例大时,灭活效果不好。铁离子10^(-5)M有同样的增效作用。铜离子协同维生素C对病毒有特殊的灭活效果,表现在它们对f_2噬菌体的灭活作用比对寄主菌的灭活作用强。维生素C与铜离子反应的中间产物是灭活噬菌体的活性物质,这种活性物质是不稳定的,可能是一种短命的自由基。

The inactivation of bacteriophages by ascorbic acid and Cu^(2+) was inves-tigated. Ascorbic acid f took the main part in inactivation of bacteriopha-ges, and Cu^(2+) exerted a catalytic effect. f_2 (RNA phage) and T_2 (DNA ph-age) were inactivated rapidly by ascorbid-acid and Cu^(2+). Ascorbic acid andCu^(2+) were effective for inactivation of phage f_2 in mollecular proportion of1:1—100:1, but ascorbic acid and Cu^(2+) in the inverse proportion were much less effective. The intermediate product from the reaction of ascorbic acidwas possibly unstable radicals which were mainly responsible for the inac-tivating effect.