聚合酶链反应检测呼吸道感染非细菌性多种病原体的研究

Detection of non-bacterial pathogens by polymerase chain reaction in respiratory tract infection

目的 评估聚合酶链反应 (PCR)方法在呼吸道感染病原学诊断方面的应用价值。方法 呼吸道感染患者 (感染组 )和健康人 (对照组 )组成研究对象 ,采集呼吸道排泌物 (鼻咽分泌物和痰 )为检测标本 ,应用聚合酶链反应(PCR)方法检测腺病毒 (ADV)、巨细胞病毒 (CMV)、柯萨奇病毒 (COX)、呼吸道合胞病毒 (RSV)、肺炎衣原体 (CP)、沙眼衣原体 (CT)、肺炎支原体 (MP)和解脲支原体 (UU)。结果 感染组的阳性检测结果分别为 :ADV 11.5 %、CMV14 .5 %、COX 18.0 %、RSV 2 9.5 %、CP 34.5 %、CT 4 .5 %、MP 17.0 %、UU 5 .5 % ;除CT和UU外 ,其他病原体的结果与对照组比较 ,差异均具有统计学意义 (P <0 .0 1)。结论 PCR方法具有高特异性和敏感性 ,可以作为呼吸道感染病原学诊断方法应用 ;CP、RSV、COX、MP等微生物可能是导致呼吸道感染重要的病原体。

Objective To evaluate the value of polymerase chain reaction(PCR) in the diagnosis of respiratory tract infections.Methods The study subjects were the patients with respiratory tract infections (infected group) and healthy men (control group).Nasopharyngeal secretion and sputum were sampled for detection.Adenovirus(ADV),cytomegalovirus(CMV),coxsackie virus(COX),respiratory syncytial virus(RSV),chlamydia pneumonia(CP),chlamydia trachomatis(CT),mycoplama pneumonia(MP) and ureaplasma urealyticum(UU) were detected by PCR.Results The positive rates in infected group were ADV 11.5%,CMV 14.5%,COX 18.0%,RSV 29.5%,CP 34.5%,CT 4.5%,MP 17.0% and UU 5.5%.Compared with the control group,all the other items except CT and UU had statistic significance (P< 0.01).Conclusion PCR can be a useful way in the diagnosis of respiratory tract infections due to its higher sensitivity and specificity.CP,RSV,COX and MP may be the major pathogens of acute respiratory tract infections.