抗犬细小病毒复方制剂的研究

Study on anti-CPV composite

对山茶叶、轮叶党参、卵叶芍药、苦味西葫芦、狼毒大戟 5种中草药的提取物进行体外抗犬细小病毒 (CPV)筛选 ,并对以山茶叶、黄芪等组成的复方进行抗病毒效果评价与药理作用研究。结果表明 ,山茶叶抗CPV作用较强 ,其对CPV的抑制率为 55 2 4 % ;由山茶叶组成复方对CPV的抑制率为 63 0 4 % ,抗病毒效果明显优于单方的山茶叶和药物对照黄连 ;对CPV 50 %抑制浓度 (IC50 )为 1∶587 5 ,提示有较强抗病毒作用。由山茶叶、黄芪等组成的复方药物对大肠杆菌、沙门氏菌、金黄色葡萄球菌有抑制作用 ,可明显增强机体免疫力。

The in vitro anti-CPV effect of the extracts of Cass nomame (sieb) honda, Codonopsis Lanaceolatabenth et Hook, Paeonia obovata maxim, Curcurbita pepo Dayanggua and Euphoriba Fisheriana steud was studied. The pharmacological action of the composite of Cass nomame (sieb) honda and Astragalus mongholicus Bunge was studied. The result showed that antiviral effect of Cass nomame (sieb) honda was apparently prior to other extracts in F81 cell and the inhibition rate of Cass nomame (sieb) extracts was 55.24%; the composite's inhibition rate was 63.04% and the anti-CPV effect was prior to Cass nomame (sieb) honda's and Jeffersonia dubia benth's, the IC_(50)was (1∶587.5.) The composite was effective on CPV and could inhibite drug-resistance trains of E coli, S cholera sais, S aureus and Mycoplasma gallinarum, enhance lymphocyte transformation.