重组鸡IL-2增强鸡四联灭活苗免疫效果的研究

Immune enhancing effect of recombinant IL-2 on a four in one oil adjuvant inactivited vaccine

使用在原核表达系统中表达的重组鸡白细胞介素 2 (IL 2 )蛋白 ,经过初步的分离纯化 ,与鸡新城疫 禽流感 法氏囊 传染性支气管炎四联油乳剂灭活苗同时使用 ,检测其对疫苗的免疫增强效果。试验共分 5组 ,每组 1 0只鸡。其中对照组只注射四联油乳剂灭活苗 ;4个试验组 ,在注射疫苗的同时分别注射重组鸡IL 2蛋白 0 0 5mg、 0 0 1mg、 0 1 5mg、 0 2 0mg。试验组与对照组同时各注射新城疫等多联油苗 0 5mL。采用HA、HI试验测定鸡新城疫、禽流感抗体滴度。结果表明 :重组鸡IL 2对该四联油乳剂灭活苗有较强的免疫增强作用。以第 3、 4组的增强效果较为明显 ,其中对鸡新城疫病毒和禽流感抗原的抗体效价 ,与对照组相比分别提高 2 42 8和 2 2 3 0个滴度。重组鸡IL 2在使用的过程中 ,没有发现任何毒副作用 ,证明是一种安全高效的免疫增强剂。

Recombinant IL-2 expressed in prokaryotic was injected with oil vaccine to study the effects on four in one oil adjuvant inactivited vaccine.The experiment had five groups,ten chickens for each group.The control group was immunized only with oil adjuvant vaccine,the other four groups were injected with IL-2 at dose of 0.05 mg,0.10 mg,0.15 mg,0.20 mg, respectively at the same time. The experiment groups and the control group were all inoculated with 0.5 mL oil adjuvant vaccine , respectively. The antibody titers of newcastle disease and avian influenza were detected by HA and HI assay. The results indicated that the recombinant IL-2 had strong effect on oil adjuvant vaccine, and the titers of antibodies increased by 2.4~2.8 titers and 2.3~3.0 titers, respectively.During the whole experiment periods, the toxic effect was not found.The results confirmed that recobmbinant IL-2 was effective in enhancing the immune antibody titers.