摘要
目的 观察不同 p H值 N- 2 -羟乙基哌嗪 - N′- 2 -乙磺酸 ( N- 2 - hydroxyethyl piperazine- N′- 2 - ethanesulfonicacid,HEPES) -重碳酸盐缓冲液 ( Krebs- Henseleit,KH)复灌液对缺血 -再灌注未成熟心肌和心肌间质的影响。 方法采用 L angendorff离体灌注模型将 2 4只大白兔分为 3组 ,对照组 ( n=8)、缺血 -再灌注组 ( n=8)和酸性灌注组 ( n=8) ,观察其左心室功能恢复率、心肌含水量 ( MWC)、血清肌酸激酶 ( CK)、乳酸脱氢酶 ( L DH)漏出率 ,心肌组织三磷酸腺苷 ( ATP)、丙二醛 ( MDA)含量和超氧化物歧化酶 ( SOD)活性 ,心肌羟脯氨酸 ( HP)和血清内皮素 ( ET)含量等指标。结果 酸性灌注组在左心室功能恢复率、ATP含量、SOD活性、HP含量等方面均优于缺血 -再灌注组 ( P<0 .0 5 ) ,在MWC、MDA含量、CK、L DH漏出率和 ET含量等均低于缺血 -再灌注组 ( P<0 .0 5 )。 结论 p H反常是缺血 -再灌注损伤的重要原因 ,复灌初期应用梯度酸性 HEPES- KH液有助于未成熟心肌和心肌间质的保护。
Objective To investigate the protection effects of different pH N 2 hydroxyethyl piperazine N′ 2 ethanesulfonic acid (HEPES) Krebs Henseleit (KH) reperfusate solutions on immature myocardium and myocardial interstitium. Methods Isolated perfused Langendorff model from immature rabbit hearts were performed. Twenty four rabbits were divided into control group, ischemia reperfusion group and acidic reperfusate group. The left ventricular function recovery, myocardial water content(MWC), lactate dehydrogenase (LDH) and creatine kinase (CK) leakage, malondialdehyde(MDA) and adenosine triphosphate (ATP) content, superoxide dismutase(SOD) activity , hydroxyproline(HP) and endothelin(ET) content were tested. Results The left ventricular functional recovery, ATP content, SOD activity and HP content in acidic reperfusate group were higher than those of ischemia reperfusion group( P <0.05).MWC, MDA content, CK and LDH leakage, and ET content in acidic reperfusate group were lower than those of ischemia reperfusion group( P <0.05). Conclusion These results suggest pH paradox might be one of important mechanisms for immature myocardium and myocardial interstitium ischemia reperfusion injury,and acidic perfusate, at the beginning of reperfusion ,might attenuate pH paradox and ameliorate functional recovery in isolated perfused immature rabbit hearts.
出处
《中国胸心血管外科临床杂志》
CAS
2004年第2期131-133,共3页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
