液体培养基在水稻花药培养中的应用研究

Application of liquid medium at anther culture in rice

采用两种培养方式(液体培养基诱导法、固体培养基诱导法)对水稻花药进行培养,结果表明,液体培养基诱导法与固体培养基诱导法培养时间相当,但液体培养基诱导法的出愈率及绿苗分化率均提高10％左右,由此认为,液体培养基诱导法是提高水稻花药培养效率的可行方法。

Different approaches (liquid medium induction and solid medium induction) were applied to culture rice anthers , the results showed the liquid medium induction was similar to the solid in cultural time, but the liquid culture could increase about 10% in the ratio of callus induction and freqency of green plantlet differentiation. Therefore, the method was a feasible way of increasing anther culture efficiency in rice.