昆明地区非洲菊疫病的PCR技术检测

Detection of PCR on Gerbera jamesonii Phytophthora in Kunming

以PCR技术检测非洲菊疫病比传统方法迅速,检测效率明显。从3个模板DNA源(病株、病株栽培土、通过病株槽的灌溉水)提取的目的DNA均可通过PCR技术检测出隐地疫霉菌的存在。当灌溉水中隐地疫霉的孢子浓度达1×102/ml的数量级时,便可以通过PCR技术检测出来。

Using PCR technology to measure Gerbera jamesonii Phytophthora is more rapid and efficiency than the traditional method. Purpose DNA, drawn from three template DNA sources (diseased plant, diseased plant culture soil, irrigating water through diseased plant tank) , all can detect Phytophthora existence through PCR technology. When the density of spore is up to the quantity magnitude of 1×10~2 /ml in irrigating water, Gerbera jamesonii Phytophthora can be detected with PCR technology.