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小麦籽粒硬度Friabilin蛋白SDS-PAGE生化标记研究 被引量：1

An improved SDS-PAGE method as a biochemical marker for grain hardness in common wheat
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摘要 籽粒硬度是重要的小麦(T.aestivum L.)品质性状。水洗淀粉表面分子量约15 KDa的Friabilin蛋白是这一性状的生化基础。用单粒谷物特性仪(SKCS)和改进的聚丙烯酰胺凝胶电泳(SDS-PAGE)分析了104份国内外冬小麦品种(系)的籽粒硬度与Friabilin表达量的关系,结果表明,Friabilin谱带相对表达量与籽粒硬度显著相关,Friabiiin谱带强,籽粒硬度数值低,Friabilin谱带弱,籽粒硬度数值高;用Friabilin蛋白表达量判断籽粒软硬的准确率为86.5％,其相关系数为-0.66,达到1％的显著水平。因此,改进的Friabilin蛋白SDS-PAGE技术可用于小麦籽粒硬度生化标记的辅助选择。 Grain hardness is important quality trait of common wheat (T.aestivm L) . A starch surface associated protein, friabilin (Mr15KDa), is a putative marker for softness. 104 varieties and advanced lines were used to measure kernel hardness and friabilin level by Single Kernel Characterization System (SKCS) and improved SDS-PAGE. The results show that high level of friabilin protein in soft wheat and relatively low level in hard wheat, with correlation coeffi-cience-0.66 for the tested materials. An improved SDS-PAGE method as a biochemical marker assistant selection for grain hardness in common wheat.
作者 郭世华 刘丽 于亚雄 何中虎
机构地区 内蒙古农业大学农学院 云南省农业科学院粮食作物研究所 中国农业科学院作物育种栽培研究所
出处 《西南农业学报》 CSCD 2004年第B05期14-17,共4页 Southwest China Journal of Agricultural Sciences
基金 国家自然科学基金(30260061) 云南省自然科学基金(2003C0027Q) 云南省科技攻关项目(2001NG18)
关键词 小麦 籽粒硬度 Friabilin蛋白 SDS-PAGE 品质性状 生化标记 相关系数 common wheat grain hardness Friabilin SDS-PAGE
分类号 S512.103 [农业科学—作物学]
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参考文献14

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二级参考文献3

共引文献15

同被引文献11

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西南农业学报
2004年 第B05期

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