大鼠实验性高血压和脑出血乳头体和正中隆起心钠素含量的改变

The Concentration of Atrial Natriurebic Factor in the Mammillary Bodies and the Median Eminence of Experimental and Spontaneous Hypertensive and Cerebral Hemorrhaged Rabs

乳头体是突出于下丘脑底面的半球形核团、是脑内血管最密集、血液供应最丰富的区域之一,也是神经病学和解剖学较少涉足的领域。心钠素(Atrial Natriuretic Factor.ANF)是近几年来新发现的血管调节多肽,已有人发现乳头体内侧核含有许多ANF免疫活性神经元,但尚未见乳头体ANF含量的定量分析及病态下乳头体ANF含量变化的报告。本实验分三组对实验性脑出血原发性高血压大鼠(Spontaneously Hypertensive Rat,SHR)、对照组SHR和Wistar大鼠的乳头体和正中隆起ANF进行了定量观察。结果发现:随着血压升高,乳头体ANF含量呈动态变化,出血组SHR乳头体ANF含量为93.98pg/mg,对照组SHR为68.82/mg.Wistar大鼠为30.09pg/mg,出血组SHR和Wistar大鼠乳头体的ANF含量有显著差异(t=2.40,p<0.05),而三组大鼠正中隆起处ANF含量相对稳定。实验结果表明,乳头体ANF以某种方式参与了对血压的调节,高血压、高盐饮食对乳头体ANF含量都会产生明显影响,但具体调节方式和调节途径目前还不清楚,而正中隆起ANF与血管调节活动无关。可能主要参与对垂体前叶功能的调节。

The mammillary body, a pair of hemispheric tissue located at the base of brain behind hypothalamus, was known to have abundant vessels and ample supply of blood. Recently Atrial Natriuretic Factor (ANF), a peytide involoved into the central regulation on blood pressure, was reported. It was also noticed that there were many ANF immunoreactive neurons in the medial nucleus of mammillary body (MB). but upto now few report dealing with the ANF concentration and its change under pathological status was ever published. The content of ANF in MBs of rats from 3 experimental groups namely, hemorrhegd and contral SHR, and Winstar rats, were measured. The concentration of ANF was revealed the much higher in SHR with cerebral hemorrhage than contral (93.98 pg/mg vs 68.82pg/mg, p0.05) and Wistar (30.09pg/mg). However no significant difference was detected among the ANF levels in median eminences of the three groups. Judged from present study, therefore, the ANF in MB was believed to be relcassed into circulation to regulate in certain way the blood preessure, and salt in diet might affect the concentration; while the ANF in median eminence was probably involved mainly in the regulation of pituitary function.