HPLC法测定重型颅脑损伤患者尼莫地平的血药浓度

Determination of plasma nimodipine concentrations in severe craniocerebral trauma patients

目的 建立重型颅脑损伤患者血浆尼莫地平浓度的HPLC测定方法。方法 以地西泮为内标;血浆样品经甲醇沉淀蛋白,上清液氮气吹干,流动相复溶等处理后进样;色谱柱为Nova-Pak C18 (3.9 mm×150 mm,4 μm);流动相为甲醇-水(48:52),流速为1.0 mL·min-1;检测波长为238 nm。结果 色谱峰分离良好,无干扰。线性范围为2.5-250 μg·L-1;检测限为2.5 μg·L-1。平均回收率100.2％;日内及日间精密度均小于10％。利用本法测定了服用尼莫地平的重型颅脑损伤患者的血药浓度。结论 本法可用于患者尼莫地平血药浓度的监测,方法简便、灵敏、准确。

OBJECTIVE To establish a high performance liquid chromatography (HPLC) method to determine plasma nimodipine concentrations in severe craniocerebral trauma patients. METHODS The plasma samples, added diazepam as the internal standard, were deposited down the protein with methanol. The supernatant was evaporated by N2 at 40℃. The remains were dissolved with 100 μL mobile phase solution; 20 μL of them was injected to the sample; Nova-Pak C18 (3. 9 mm×150 mm, 4 μm) column was used (mobile phase: MEOH: H2O=48:52; flow rate 1. 0 mL·min-1). Ultraviolet detection wavelength was 238 nm, RESULTS The chromatography was good and not interferred by the components of the plasma. The linear range was 2. 5-250 μg·L-1. The lowest detectable limit was 2. 5 μg·L-1. The average recovery was 100. 2%. RSD values of within-day and between-day was not over 10%. Eight patients' samples were determined with this method after intra-venous drip of nimodipine. CONCLUSION This study provides a simple, accurate, reliable method for clinical pharmacokinetic studies as well as determining plasma nimodipine concentration in severe craniocerebral trauma patients.