中性糖对古糖酯含量测定的影响

Comparison of Three Methods for Content Determination of Sulfated Polyguluronic Acid Ester

采用硫酸 -苯酚法、硫酸 -咔唑法和硫酸 -间羟联苯 3种方法分别对古糖酯的含量测定进行了研究 ,并考察了 3种方法中加入 8种单糖即阿拉伯糖 (Ara)、半乳糖 (Gal)、葡萄糖 (Glc)、果糖(Fru)、木糖 (Xyl)、甘露糖 (Man)、鼠李糖 (Rha)和岩藻糖 (Fuc)后对古糖酯含量测定的影响。结果表明 3种方法测定古糖酯含量时 ,其吸收值与古糖酯浓度 (在 0 .1～ 0 .5 mg/ m L 范围内 )都呈良好的线性关系 ,不同的中性糖对 3种测定方法影响不同。样品回收率亦不相同。

Three determination methods,i.e.sulfate-phenol, sulfate-carbazole and sulfate-3-Phenylphenylol for uronic acid, were applied to determine the content of sulfated polyguluronic acid ester (SPGE). And different neutral sugars i.e. Ara, Gal, Glc, Fru, Xyl, Man, Rha and Fuc,were added to examine their effects on the determination of SPGE.The results show that the absorbance is in direct ratio with the concentration of SPGE between 50 and 500μg/mL. Different neutral sugar in SPGE can influence the results in a different degree.With the refined SPGE as a criterion,there is little differences in the recovery coefficient for the three methods.