水牛实验感染大片吸虫及ELISA检测特异性抗体的变化

FASCIOLA GIGANTICA EXPERIMENTAL INFECTION IN BUFFALO AND DETECTION OF SPECIFIC ANTIBODIES USING ELISA

目的 本试验为了对水牛感染大片吸虫的情况进行了解。方法 选 10头 6月龄水牛 ,分成 2组 ,每组 5头 ,A组对照 ,B组试验 ,每头经口感染 2 5 0个大片吸虫囊蚴。结果 水牛感染大片吸虫后的收虫率为 16 .2± 8.0 % ,在感染后第 13～ 14周检出虫卵。抗大片吸虫分泌排泄产物 Ig G水平从感染后第 2周明显升高 ,17周时达到高峰。结论 试验证实水牛对大片吸虫感染很敏感 ,EL

Fasciola gigantica experimental infection in buffalo was studied. 16.2±8.0% of the infection dose was recovered as adult flukes from infected animals at necropsy. Buffalo seemed to be susceptible to F.gigantica infection. The prepatent period was of 13～14 weeks post infection (WPI). Anti F.gigantica Excretory Secretory Products (FgESP) IgG level increased significantly from 2 WPI and the IgG responses to FgESP by ELISA displayed a peak at 17 WPI. Detection of specific IgG by ELISA could be used for early diagnosis of fasciolosis in ruminants.