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压电免疫传感器在梅毒检测中的研究 被引量：4

Piezoelectric Immunosensor for the Detection of Treponema Pallidum
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摘要 用巯基丙酸 (MPA)为基层物质在压电石英晶体表面形成单分子膜 ,经过N_乙基_N′_(3_二甲氨基)丙基碳化二亚胺盐酸(EDC)和N_羟基琥珀酰亚胺(NHS)活化后固定梅毒抗原TpAg ,制成免疫传感器检测不同浓度的标准抗体溶液。并考察了传感器的响应特性和免疫反应动力学特性 ,结果表明这种免疫传感器是临床检测的有效工具 ,有巨大的应用潜力。 A piezoelectric immunosensor for the detection of treponema pallidum has been developed.On self-assembled monolayers of3-mercaptopropionic acid(MPA)on the gold surface of a piezoelectric quartz microbalance,antigen of treponema pallidum was immobilized via the EDC-NHS activation process(N- ethyl- N′-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide).The sensor was calibrated by standard antibody solutions of different concentrations.The characteristics of the resoˉnant frequency response as well as the immunoreaction kinetics have been studied.Our results demonˉstrate that piezoelectric immunosensor is an effective tool in clinic assay and has tremendous application poˉtential.
作者 陈志敏 杨连生 刘仲明 刘芳
机构地区 华南理工大学食品与生物工程学院 广州军区广州总医院医学实验科
出处 《分析测试学报》 CAS CSCD 北大核心 2004年第3期39-42,共4页 Journal of Instrumental Analysis
基金 广东省重点攻关项目 (2km04301s) 全军医卫科研基金项目 (98M038)
关键词 压电免疫传感器 自组装单层膜 固定化 梅毒 抗原/抗体 Piezolelectric immunosensor Self-assembled monolayers Immobilization Treponema pal-lidum Antigen/antibody
分类号 TP212.3 [自动化与计算机技术—检测技术与自动化装置] R377.1 [医药卫生—病原生物学]
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二级参考文献11

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共引文献19

同被引文献74

  • 1郑和平,欧志英,胡玉山,黄进梅,李美玲,吴兴中,曾维英,潘慧清.梅毒螺旋体的巢式PCR检测与基因分型[J].中华皮肤科杂志,2005,38(9):546-548. 被引量：22
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  • 7SCHOFIELD A L, RUDD T R, MARTIN D S, et al. Real-time monitoring of the development and stability of biofilms of Streptococcus mutans using the quartz crystal microbalance with dissipation monitoring[ J]. Biosens Bioelectron, 2007,23 (3):407.
  • 8SU X L, LI Y. A QCM immunosensor for salmonella detection with simultaneous measurements of resonant frequency and motional resistance [ J]. Biosens Bioelectron, 2005, 21 ( 6 ) :840.
  • 9MAO X,YANG L, SU X L, et al.A nanoparticle amplification based quartz crystal microbalance DNA sensor for detection of escherichia coli O157:H7 [J]. Biosens Bioelectron, 2006,21(7) : 1175.
  • 10ZUO B, LI S, GUO Z, et al. Piezoelectric immunosensor for SARS-associated coronavirus in sputum[J]. Anal Chem, 2004,76 (13) : 3536.

引证文献4

二级引证文献37

分析测试学报
2004年 第3期

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