白芍总苷的薄层色谱指纹图谱实验研究

TLC Fingerprinting Analysis of Total Glycosides of Paeony (TGP)

目的为考察商品白芍总苷质量的稳定性和一致性,建立薄层色谱指纹图谱。方法硅胶60预制板(Merck);氯仿-醋酸乙酯-甲醇-甲酸(40∶5∶10∶0.2)为展开剂;5%香草醛硫酸溶液喷雾显色,80℃加热至斑点显色清晰。结果10批样品的薄层色谱指纹图谱所形成的全谱图像显示批间产品有很高的相似性。结论薄层色谱与液相色谱在指纹图谱的应用中各有特色,互为补充。

Objective To establish TLC fingerprint of TGP for assessing the quality consistency of batch- by- batch of commercial product. Methods Silica gel 60 precoated plate (Merck) was used. Solvent system included chloroform- ethyl acetate- methanol- formic acid (40 ∶ 5 ∶ 10 ∶ 0.2); ∶ 5 % vanillin- sulfuric acid reagent was used as visualizing reagent and the heating temperature at 80 ℃ . Results Consistency among ten batches of TGP was demonstrated by TLC fingerprint image and the profiles generated by digital scanning. Conclusion TLC fingerprint of different batches of TGP confirms that TLC is also a powerful tool for developing chromatographic fingerprint, it can be used as a complementary technique for HPLC. 