激素对沙漠玫瑰愈伤组织培养的影响

The effect of hormone on Callus Culture of Desert Rose Adenium obesum

用沙漠玫瑰无菌苗充分伸展的叶片诱导出愈伤组织,进行愈伤组织的继代培养,以获得适于悬浮培养的愈伤组织。比较了不同植物生长调节剂及其配比在沙漠玫瑰离体培养中的效应,认为叶片接种到BA1～2mg/L+NAA0.2～0.4mg/L的MS培养基中,愈伤组织的诱导效果最好;愈伤组织继代培养时,含2,4-D1～3mg/L或2,4-D1～2mg/L+BA0.5～1.0mg/L的MS培养基的愈伤组织继代培养效果较好。

In order to obtain the callus for suspension culture, studies were made on the induction and subculture of callus in Adenium obesum and the effects of plant regulators used were tested in the whole process. It was revealed that using the medium with BA 1～2 mg/L+NAA 0.2～0.4 mg/L to induce callus achieved the best results. The medium with 2, 4-D 1～3 mg/L or 2, 4-D 1～2 mg/L+BA 0.5～1.0 mg/L was the optimal mixture on which the callus was yellow and loose, and suitable for suspension culture.