抗体固载于TiO_2多孔膜的压电免疫型细菌传感器

Piezoelectric immunosensor for detection of bacteria by immobilizing antibodies on TiO_2 porous films

在镀银的压电石英晶体上沉积一层TiO2纳米粒多孔膜,用3 氨丙基三乙氧基甲硅烷将其活化后,借助于戊二醛实现了特异性抗体(抗肠道沙门氏菌抗体)在压电石英晶体上的有效固载,并用于肠道沙门氏菌的快速检测。其检测下限为4×104cells mL,检测时间为30min。可用于价格低廉的镀银石英晶体,有望成为开发一次性压电型免疫检测探头的有效方法。

Nanocrystalline TiO_2 porous films were coated on the surface of silver-plated piezoelectric crystal, and used for the immobilization of antibodies (anti-Salmonella enteritidis antibodies) after activation by (3-aminopropyl)trimethoxysilane and glutaraldehyde. The piezoelectric immunosensor developed by the present modification method was used to detect Salmonella enteritidis. A detection limit of 4×10~4 cell/mL and an assay time of 30 min were achieved. Considering its simplicity, reproducibility and cost, it is feasible to develop disposable piezoelectric crystal detectors for immunological test.