草原红牛微卫星DNA多态性的研究

Study on Polymorphisms of Red Steppe Cattle by Microsatellites

为了更好地培育和改良肉用草原红牛 ,利用草原红牛、夏洛莱牛、西门塔尔牛、利木赞牛、蒙古牛基因组DNA ,用 8对微卫星引物进行PCR扩增 ,并将扩增产物置于 1 5 %琼脂糖电泳进行观察 ,如有所需的带则转入 8%聚丙烯酰胺变性凝胶 (PAGE)中电泳分离 ,绘制指纹图谱。

Eight microsatellites were analysed for polymorphisms in Charolais, Limousin, Simmental, Red Steppe Cattle, Mongolian Cattle For the purpose of founding the staining methds for revealing microsatellite polymorphism of domestic animal, PCR amplification was carried out with 8 pairs of microsatellite promers using genomic DNA of Red Steppe Cattle, Charolais,Limousin,Simmental et al Amplified products were separated by denaturing pollyacylamide gel elextrophoresis It was thought that Microsatellites DNA was available method Genetic relations between Red Steppe Cattle and Charolais et al were stated at molecular biotechnique level