摘要
利用优化了的银染mRNA差异显示技术 (DD PCR) ,比较了出生后 40日龄、60日龄和 12 0日龄三个不同生长阶段的绵羊相同部位的皮肤中总RNA的差异表达。结果表明 :同一个体不同日龄的绵羊存在着表达量不同的差异表达基因 ,并初步筛选出了 8条重复性好的差异cDNA片段 ,其中 1条表达量不同的差异片段存在于 40日龄 ,5条存在于 60日龄 ,2条为 12 0日龄所特有。
using optimized the silver straining mRNA differential display techinques,total RNA was compared in skin of three different growth phases(after birth 40,60,120 days) of sheep the result showed that there are differential expressive genes in skin of different growth phases of sheep,and preliminavily isolate eight high repeatabilith differential cDNA bands,one of them differential bands exist in 40 days,five of them exist in 60 days,two of them exist in 120 days This test will provide scientific reference basis for studying effecting character of wool associated with genes expression in skin
出处
《中国草食动物》
2004年第3期11-14,共4页
China Herbivores
