摘要
A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide（CTAB） was used to reverse electro-osmotic flow in order to shorten the analysis time. β-CD was added to optimize the separation further. The effects of separation potential,concentration of CTAB,buffer electrolyte and β-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids were separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H3BO3/0.10 mmol/L CTAB/0.25 mmol/L β-CD（pH=11.0） at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991—0.999 in the linear range of 1.00×10 -6 —1.00×10 -3 mol/L,the limit of detection are 0.17—0.94 μmol/L（S/N=3）. [WT5HZ]
A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide(CTAB) was used to reverse electro-osmotic flow in order to shorten the analysis time. β-CD was added to optimize the separation further. The effects of separation potential,concentration of CTAB,buffer electrolyte and β-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids were separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H_3BO_3/0.10 mmol/L CTAB/0.25 mmol/L β-CD(pH=11.0) at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991—0.999 in the linear range of 1.00×10 -6 —1.00×10 -3 mol/L,the limit of detection are 0.17—0.94 μmol/L(S/N=3). [WT5HZ]
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第6期1037-1039,共3页
Chemical Journal of Chinese Universities
基金
广东省自然科学基金重点项目 (批准号 :0 2 180 8)资助
关键词
反向毛细管电泳
高频电导检测
非接触式电导检测
氨基酸
Reversed capillary electrophoresis
High frequency conductivity detection
Contactless conductivity detection
Amino acids
