高效液相色谱法测定苦参素胶囊中苦参素含量

Determination of Oxymatrine in Oxymatrine Capsule by HPLC

目的 建立苦参素含量的高效液相色谱测定方法。方法 采用高效液相色谱法 ,色谱柱为Shim -packVP -ODSC18( 4 .6mm× 2 0 0mm ,5 μm) ,流动相为甲醇 -水 -冰醋酸 ( 80∶2 0∶1 ) ,流速为 1 .0mL min ,柱温为 30℃ ,检测波长为 2 2 0nm ,进样量为 2 0 μL。结果 苦参素浓度在 ( 1 0 .1～ 5 0 .7) μg mL内 ,峰面积与进样量呈良好线性关系 ,r =0 .9998,平均回收率为 1 0 0 .2 %。结论 本文建立的方法简便 ,重现性好 ,可用于苦参素的含量测定。

Objective To develop a HPLC method for the determination of oxymatrine. Method We used a RP-HPLC method with Shim-pack VP-ODS column (5 μm, 4.6 mm×200 mm), and the mobile phase was the mixture of methanol-water acetic acid glacial (80∶20∶1) at the flow rate of 1.0 mL·min -1 . The column temperature was 30℃, the UV detection wavelength was 220 nm. Result The callbration curve was linear (r=0.999 8) in the range of (10.1～50.7)μg·mL -1 for oxymatrine. The mean recovery was 100.2%. Conclusion This method was simple, selective and reproducible for the determination of oxymatrine in oxymatrine capsule.