体外培养人眼晶状体上皮细胞整合素α5的表达

Expression of integrin α5 in cultured human lens epithelial cells in vitro

目的 探讨体外培养的人眼晶状体上皮细胞整合素α5的表达。方法 采用免疫荧光细胞计数检测法 ,对 2 0例先天性白内障患者术中的环形撕裂前囊膜进行原代培养并传代 ,取 2～ 3代细胞进行检测整合素α5的表达。结果 体外培养人眼晶状体上皮细胞整合素α5的表达阳性率平均为 93 .3 0 % ,阴性率为 6.70 %。结论 体外培养人眼晶状体上皮细胞高表达整合素α5 ,其可能在后发性白内障的发生和发展过程中发挥作用。

Objective To investigate the expression of integrin α5 in cultured human lens epithelial cells in vitro. Methods Twenty patients with congenital cataract had undergone continuous circular capsulorhexis and the lacerated anterior cyst membrane cells were taken out for primary culture and cultured for 2～3 generations in vitro. Then an immunofluorence flow cytometer technique was used to determine the expression of integrin α5 in the 2～3 generation anterior cyst membrane cells. Results The mean positive rate was 93.30% and the negative rate was 6.70% in integrin α5 in cultured human lens epithelial cells. Conclusion High expression of integrin α5 were found in cultured human lens epithelial cells in vitro, it might play a role in the genesis and development of after-cataract.