蛋白质的反射散射光度研究

Study on the Determination of Protein by Diffuse Reflectance Spectroscopy

研究了微晶石蜡吸萃蛋白质 偶氮胂Ⅲ 镝(Ⅲ)复合物的条件,并设计了固相反射散射分光光度直接测定石蜡相中蛋白质的方法。所拟定的方法有较好的选择性,可以用于实际生物样品中蛋白质含量的测定。

A method for the determination of protein by diffuse reflectance spectroscopy after the extration of the complex of protein with arsenazo Ⅲ-Dy(Ⅲ) with microcrystalline wax has been studied. The method has been used successfully for the determination of microamounts protein in biological sample.