p16蛋白及其基因在原发性肝细胞癌中的表达缺失及意义

A study on p16 protein expression and p16 gene deletion in primary hepatocellular carcinoma

目的 :通过p1 6蛋白及其基因在原发性肝细胞癌中的表达缺失研究 ,探讨p1 6基因与肝癌的关系 .方法 :收集我院原发性肝癌 32例 ,用免疫组织化学SP法、聚合酶链式反应(PCR)方法分别检测肝癌及癌旁组织中p1 6蛋白的表达及其基因的缺失情况 .结果 :32例肝癌中 ,p1 6蛋白表达缺失率为6 2 .5 % (2 0 / 32 ) ,癌旁组织中p1 6蛋白表达缺失率为 3.1 3%(1 / 32 ) ,两者差异有显著意义 (P <0 .0 1 ) .p1 6蛋白的表达与肝癌分化程度关系密切 (P <0 .0 1 ) .p1 6基因在癌组织中的缺失率 2 5 % (8/ 32 ,均为p1 6蛋白表达缺失者 ) ,在癌旁组织为3.1 3% (1 / 32 ) ,相差显著 (P <0 .0 5 ) .结论 :p1 6基因缺失与肝癌发生关系密切 ,提示p1 6蛋白可能是预测患者预后的一个指标 。

AIM: To study the p16 protein expression and p16 gene deletion in hepatocellular carcinoma (HCC) and to evaluate the relationship between p16 gene and HCC. MEHTODS: The expression of p16 protein and the deletion of p16 gene were detected by immunohistochemistry and polymerase chain reaction (PCR) in 32 cases of HCC. RESULTS: The loss of p16 protein expression was observed in 20 cases of HCC (62.5%, 20/32) and in 1 case of tumor adjacent tissues (3.13%, 1/32), respectively ( P < 0.01). The loss of p16 protein expression was closely correlated with the poor histological differentiation of the tumor ( P < 0.01) . The deletion rate of p16 gene in HCC (25%,8/32) was significantly different from that in tumor adjacent hepatic tissues (3.13%, 1/32, P <0.05). CONCLUSION: There is a close relationship between hepatocarcinogenesis and p16 gene inactivation. The expression of p16 protein can be used as an index in predicting the prognosis.