阿黑皮素原相关肽对大鼠离体黄体细胞孕酮生成的影响

Influences of pro-opiomelanocortin related peptides on progesterone secretion by rat luteal cells in vitro

采用体外孵育大鼠黄体细胞的方法,观察了β-EP,β-LPH,α-MSH 和 ACTH 等阿黑皮素原(POMC)相关肽对孕酮分泌的影响,结果表明:β-EP(10^(-6),10~7,10^(-6)mol/L)可明显促进黄体细胞基础孕酮分泌,呈剂量-效应依赖关系,孕酮含量分别由对照组36.56±8.63pmol/10~6细胞上升为52.90±18.31(P<0.01),73.86±18.21和80.37±9.86pmol/10~6细胞(P<0.01);β-EP(10^(-7)mol/L)还能加强hCG 致孕酮生成作用,孕酮含量由47.30±11.57上升为63.34±9.32pmol/10~6细胞(P<0.01);纳络酮(10^(-6)mol/L)可完全阻断β-EP 的作用。与β-EP 不同,β-LPH(10^(-6)mol/L)抑制基础和 hCG 诱导的孕酮分泌;α-MSH(10^(-6)mol/L)对基础孕酮分泌无明显影响,但能加强 hCG 致孕酮生成作用;ACTH 对大鼠黄体细胞孕酮分泌无明显影响。实验显示:某些 POMC 相关肽,尤其是β-EP 可能以旁分泌的方式参与黄体细胞孕酮分泌的调节。

The effect of pro-opiomelanocortin(POMC) relat- ed peptides(β-EP,β-LPH,α-MSH,and ACTH) on pro- gesterone secretion was investigated within vitro incu- bation of rat luteal cell.It was shown that β-EP(10^(-6), 10^(-7),10^(-6)mol/L) significantly stimulated basal proges- terone secretion in a dose-dependent manner.Further- more,β-EP(10^(-7)mol/L) augmented hCG induced pro- gesterone secretion.Naloxone(10^(-6) Stool/L) could com- pletedly inhibit increment of progesterone secretion in- duced by β-EP.In contrast to β-EP,both basal and hCG induced progesterone secretion were decreased by β-LPH(10^(-6)mol/L),α-MSH(10^(-6) mol/L) could aug- ment hCG induced progesterone secretion,but it had no obvious effect on basal progesterone secretion. ACTH did not affect progesterone secretion by rat luteal cells in vitro.The present experiment indicated that some of the POMC related peptides existing in the rat ovary,particularly β-EP,might be involved in the modulation of progesterone secretion by corpus luteum cells,possibly in a psracrine manner.