PTEN、CDK4蛋白在子宫内膜癌中的表达及其临床意义

Expression of PTEN,CDK4 protein in endometrial carcinoma and its clinical significance

目的 :探讨PTEN、CDK4蛋白表达与子宫内膜癌 (EC)的发生、发展及复发的关系。方法 :采用免疫组织化学S -P法 ,测定 5 0例EC、12例不典型增生子宫内膜及 10例正常子宫内膜中PTEN、CDK4蛋白的表达情况。结果 :1.PTEN蛋白在EC中的表达明显低于正常子宫内膜及不典型增生子宫内膜中的表达 (P <0 .0 5 ) ,且与EC肌层浸润程度相关。 2 .CDK4蛋白在EC和不典型增生子宫内膜中的表达明显高于正常内膜组 ,且与EC的临床分期、组织学分级、肌层浸润程度及有无复发显著相关。结论 :PTEN蛋白表达下降或缺失及CDK4的过度表达可能在EC的发生、发展、浸润及复发中具有重要作用。

Objective:To study the relationship between cyclin dependent kinase 4 (CDK4),PTEN protein expression and carcinogenisis,progression and recurrence of endometrial carcinoma.Methods:A specific and sensitive immunohistochemical S-P assay was used to detect the expression of PTEN and CDK4 in paraffin-embedded sections of 50 cases of endometrial carcinoma,12 atypical hyperplasia of endometrium and 10 normal endometrium.Results:1.The positive immunostainning rates of PTEN in endometrial carcinoma,atypical hyperplasia of endometrium and normal endometrium were 56.00%,91.00%,100.00%,respectively;and the difference was statistically significant between carcinoma and normal endometrium or atypical hyperplasia of endometrium(P<0.05).The expression of PTEN correlated with muscle invasion(P<0.05),but not with histological grade or recurrence.2.The positive rate of CDK4 nuclear expression in carcinoma and in atypical hyperplasia of endometrium was significantly higher than that in normal endometrium(P<0.05).The nuclear positive expression and overexpression rates of CDK4 were significantly higher in stage Ⅱ～Ⅲ,moderately poorly differentiated(G 2-G 3)carcinoma,>1/2 muscle invasion cancer and in recurrence cases.Conclusion:The results suggest that the aberrant expressions of PTEN and CDK4 are closely related to the occurrence,development and recurrence of endometrial carcinoma.