生长因子在实验性尿酸盐肉芽肿形成和转归中的作用

Growth factor plays a role in the forming and progressing process of experimental uric acid granulomas

的 :探讨转化生长因子 - β1(TGF - β1)与尿酸盐肉芽肿形成和转归之间的关系。方法 :采用免疫组化及原位杂交法观察 30例尿酸性肾病大鼠肾小管周围肉芽肿形成过程中TGF - β1、PCNA、α -SMA及Ⅲ型胶原在不同时段的表达情况。结果 :肉芽肿形成的整个过程中受损伤的肾小管上皮细胞及肉芽肿内单核细胞均有TGF - β1mRNA及TGF - β1蛋白和PC NA的表达。且TGF - β1的表达与肉芽肿内Ⅲ型胶原沉积程度一致。结论 :TGF - β1可能通过自分泌和旁分泌刺激尿酸盐结晶周围单核细胞原位增殖而促进异物肉芽肿形成 ,且在肉芽肿纤维化过程中发挥了重要作用。

Objective:To study the relationship between the expression of TGF-β 1 and the formation and progression of experimental uric acid granulomas in rats.Methods:The formation and progression of granulomas in 30 cases of uric acid nephropathy,the expressions of protein and mRNA of TGF-β 1,moreover PCNA,α-SMA and collagen Ⅲ were investigated with ABC immunohistochemical staining and in situ hybridization techniques.Results:The mononuclear cells in granulomas and the injured renal tubular cells expressed TGF-β 1mRNA,and synthesized protein of TGF-β 1 and PCNA.In the late stage,the degree of the expression of TGF β 1 and the deposition of collagen Ⅲ were in coincidence in granulomas.Conclusion:TGF-β 1 promotes the forming of granulomas by stimulating the proliferation in situ of mononuclear cells through autocrine and paracrine,and plays important roles in fibrosis of granulomas.