咖啡酸苯乙酯诱导人大肠癌细胞凋亡的研究

Effect of the caffeic acid phenethyl ester on the apoptosis of human colorectal cancer cell line HCT116

目的 :探讨咖啡酸苯乙酯 (caffeicacid phenethylester ,CAPE )诱导大肠癌HCT116细胞发生凋亡的作用 ,为CAPE用于大肠癌的临床治疗提供依据。方法 :采用MTT法、HE染色、AnnexinV FITC/PI双染色流式细胞术和末端脱氧核苷酸标记法(TUNEL) ,检测不同浓度CAPE作用后HCT116细胞增殖活性和细胞凋亡的变化。结果 :2 5、5 0、10 0mg/L的CAPE处理HCT116细胞 2 4h后 ,抑制率分别为12 2 0 %、2 2 44 %、3 7 86% ,细胞增殖明显受到抑制 ,呈量效依赖性 ;凋亡率分别为( 10 2 0± 0 66) %、 ( 16 60± 0 61) %、( 2 5 5 3± 3 2 7) % ,均显著高于对照组 ( 5 47± 0 93 ) % ,P <0 0 1。HE染色呈现典型的凋亡细胞形态特征。TUNEL法标记见明显的凋亡阳性细胞。结论

OBJECTIVE: To study the effect of caffeic acid phenethyl ester (CAPE) on proliferation and apoptosis of the cultured colorectal cancer cell line HCT116.METHODS: HCT116 cells were treated with CAPE at the concentrations of 2.5,5.0,10.0 mg/L.The proliferative status of HCT116 cells was measured by using methabenzthiazuron (MTT) assay. Apoptosis was detected by HE staining,Annexin V-FITC and PI double labeling and TdT-mediated dUTP-biotin nick end labeling (TUNEL).RESULTS: After HCT116 cells were exposed to CAPE (2.5,5.0,10 mg/L) for 24 h,CAPE displayed a significant growth inhibitory effect in a dose-dependent manner against HCT116 cells. Annexin V-FITC and PI double labeling and FCM analysis showed the apoptotic rates were (10.20±0.66)%,(16.60±0.61)%,(25.53±3.27)% respectively after HCT116 cells were exposed to CAPE for 24 h,which were significantly higher than that of HCT116 cells without CAPE. Typical morphological features of apoptotic cells were detected by HE and TUNEL staining.CONCLUSION: CAPE can induce apoptosis of human colorectal cancer cell line HCT116.