不同分化程度喉癌组织中bcl-2和p53的表达及其相互关系

Expressions of apoptosis bcl-2 and p53 gene in laryngeal carcinoma and relationship between bcl-2 and p53

目的 :探讨喉癌中抗凋亡基因bcl 2和突变型 p5 3的表达、相互关系及在喉癌发生机制中的作用。方法 :用免疫组化法对正常喉组织 2 7例和喉良性肿瘤 11例进行突变型 p5 3的检测 ,对喉癌 5 2例标本进行bcl 2与突变型p5 3的检测。结果 :正常喉组织突变型 p5 3免疫组化阳性检出率为 14 8% ( 4 /2 7) ,喉良性肿瘤为 18 2 % ( 2 /11) ,喉癌为61 5 % ( 3 2 /5 2 ) ,三者间差异有统计学意义 ,P <0 0 5 ;其中低分化喉鳞癌阳性检出率为 84 6% ,中分化为 73 0 % ,高分化为 15 4% ,三者间差异有统计学意义 ,P <0 0 5 ;低分化喉鳞癌bcl 2免疫组化阳性检出率为 69 2 % ( 9/13 ) ,中分化为 3 8 5 % ( 10 /2 6) ,高分化为 2 3 1%( 3 /13 ) ,三者间差异无统计学意义 ,0 1>P >0 0 5 ;喉癌中 p5 3的表达与bcl 2的表达呈明显正相关 ,0 0 2 >P >0 0 1。结论 :p5 3基因突变可作为喉部良性肿瘤向恶性转化及恶性程度增高的一个标志 ,在喉癌的发生机制上抑制细胞凋亡的bcl 2与 p5

OBJECTIVE:To investigate the expressions of anti-apoptosis bcl-2 and p53 gene in low,middle and high differentiation laryngeal carcinoma and the relationship between bcl-2 and p53.METHODS:Twenty-seven cases of normal larynx and 11 cases of the benign tumor were studied for p53 by SP(streptavidin peroxidase)methods.Fifty-two cases of laryngeal carcinoma which had been examined for p53 by the SP methods were studied for the expression of bcl-2 by the same methods.RESULTS:The positive staining rates of p53 were 14.8% (4/27) in normal larynx,18.2% (2/11) in benign tumor of the larynx and 61.5% (32/52) in laryngeal carcinoma,and there were significant differences among them,P<0.05.The positive staining rates of p53 were 84.6% in low differentiation laryngeal carcinoma, 73.0% in middle differentiation laryngeal carcinoma and 15.4% in high differentiation laryngeal carcinoma,and there were significant differences among them,P<0.05.The positive staining rates of bcl-2 were 69.2% (9/13) in low differentiation laryngeal carcinoma, 38.5% (10/26) in middle differentiation laryngeal carcinoma and 23.1% (3/13) in high differentiation laryngeal carcinoma,there were no significant differences among them,0.1>P>0.05.The expression of p53 in laryngeal carcinoma was positive correlation to that of bcl-2 by the SP methods,0.02>P>0.01.CONCLUSIONS:The high expression of p53 is a marker showing carcinoma transfer and there is a close relationship between the inhibition of cellular apoptosis bcl-2 and p53 in the pathogenesis of laryngocarcinoma.