原癌基因c-met在肺癌组织中的表达

Relationship between c-met proto-oncogene expression and its correlation with proliferation and tumor metastasis in lung cancer

目的 :研究c met蛋白、mRNA和增殖细胞核抗原 (proliferatingcellnuclearanti gen ,PCNA)在肺癌浸润和转移中的表达及意义。方法 :应用免疫组化SP法检测 62例肺癌组织及 2 9例转移灶中c met蛋白的表达和肺癌组织中PCNA的表达 ,应用原位杂交的方法检测肺癌组织中c metmRNA的表达。结果 :62例肺癌中c met蛋白和mRNA的表达与分化程度、肿瘤大小、TNM分期和淋巴结转移相关 ,P <0 0 5。淋巴结转移站数与c met蛋白的表达亦相关 ,P <0 0 5 ;且c met蛋白在淋巴结转移灶的染色强度( 0 2 186± 0 0 2 83 )远高于相应原发灶的染色强度 ( 0 13 62± 0 0 2 66) ,P <0 0 1。PCNA标记指数 (labelingindex ,LI)与TNM分期、淋巴结转移有显著的相关性 ,P <0 0 5。c met蛋白阳性表达组PCNA LI( 0 63± 0 2 5 )显著高于c met蛋白阴性表达组 ( 0 48± 0 19) ,P <0 0 5。结论 :c met能增强肺肿瘤细胞的浸润转移能力 ,并促进肿瘤细胞增殖 。

OBJECTIVE:To study the expression of c-met proto-oncogene and proliferation cell nuclear antigen (PCNA)in lung cancer,and to explore the relationship between metastatic behavior of lung cancer and the expression of c-met and PCNA.METHODS:The expressions of c-met and PCNA protein were detected with the immunohistochemical technique,and the expressions of c-met proto-oncogene mRNA were detected by using the in situ hybridization in 62 clinical specimens.RESULTS:The expressions of c-met protein and mRNA were correlated significantly with tumor differentiation,size,stage and lymph node metastasis,P<0.05.There were also significant correlation between the expression of c-met and the N stage of lymph node metastasis,P<0.05.C-met expression in lymph node metastatic site (0.218 6±0.028 3) was significantly increased compared with that in the primary site (0.136 2±0.026 6),P<0.01.There were significant correlation between PCNA-LI and tumor stage,lymph node metastasis,P<0.05.PCNA-LI was significantly higher in c-met positive tissues (0.63±0.25) than that in c-met negative tissues (0.48±0.19),P<0.05.CONCLUSION:C-met can enhance the invasive ability of the cancer cells,induce tumor cell proliferation,and may play an important role in invasion and metastasis of lung cancer.