NF-κB与PCNA在人脑星形细胞瘤中的表达

Expression of NF-κB and PCNA in Brain Astrocytomas

目的:探讨NF-κB与PCNA在人脑星形细胞瘤以及正常脑组织中的表达情况及其与病理分级之间的相互关系。方法:SP免疫组织化学方法检测77例不同级别的星形细胞瘤及正常脑组织标本NF-κBp65与PCNA的表达情况。结果:除了在星形细胞瘤Ⅲ、Ⅳ级之间无显著性差异外(P>0.05),PCNA在其余各级别星形细胞瘤中的表达有显著性差异(P<0.05),其阳性率随着肿瘤级别的增高而升高。尽管NF-κB的表达总体差异有显著性(P<0.01),但NF-κB在星形细胞瘤相邻各级别之间的表达无显著性差异(P>0.05),然而高度恶性组(Ⅲ～Ⅳ级)阳性率显著高于低度恶性组(Ⅰ～Ⅱ级)阳性率,二者有显著性差异(P<0.01)。结论:NF-κB异常表达可能通过PCNA影响肿瘤细胞增殖活性,免疫组织化学方法联合检测NF-κB与PCNA的表达情况能客观反映星形细胞瘤的增殖恶性程度及预后。

Objective: To evaluate the relationship between the expression of nuclear factor κB (NF-κB) and proliferating cell antigen (PCNA) and pathology grade in brain astrocytomas. Methods: The immunostaining of NF-κBp65 and PCNA in 67 astrocytomas and 10 cases of normal brain tissues were performed by Streptavidin-Peroxidase (SP) method. Results: The result showed the difference of PCNA expression between tumor grades were statistically significant (P<0.05) except those in group III and group IV (P>0.05). Generally, the positive rate of PCNA increased with tumor grade. Though total variance of NF-κB expression among tumor grades was remarkable (P<0.01), there were no significant difference among group I,II,III and IV (P>0.05). However, the positive rate of NF-κB was much higher in tumors with higher grade of malignancy than in those with lower grade of malignancy (P<0.01). Conclusion: The abnormity of NF-κB may increase cellular proliferation activity by PCNA, immunohistochemical analysis of PCNA combined with NF-κB may be useful not only in deciding the grade of malignancy but also in some prognostic value in astrocytomas.