酶联免疫电转移印迹技术在囊尾蚴病诊断中的应用

Application of EITB in Immunodiagnosis of Cysticercosis

目的 应用高度敏感、特异的酶联免疫电转移印迹技术 (EITB)诊断囊尾蚴病。 方法 用双向电泳法对囊液抗原进行分离、纯化 ,并通过免疫印迹 ,用囊尾蚴病患者、棘球蚴病患者和其他异源血清筛选高免疫反应性和高特异性抗原组分 ,以此建立EITB诊断方法 ,与传统的ELISA法相比 ,并比较血清和滤纸干血浸出液两种样本的敏感性。 结果 得到了等电点为 9.4,相对分子质量为 14 0 0 0和 1660 0两组特异性抗原组分 ,以此为诊断抗原建立了EITB诊断方法 ,其敏感度和特异度分别高达 92 .5 %和 10 0 % ,显著高于ELISA法。在EITB中血清和滤纸干血浸出液的敏感性相似。 结论 EITB诊断囊尾蚴病较ELISA法敏感性高。

Objective To establish a sensitive and specific method EITB for the immunodiagnosis of cysticercosis. Methods Two-dimensional electrophoresis was used to analyze and purify the cyst fluid antigen. Western blotting using the sera of patients with cysticercosis, hydatidosis, and other heteroserum was used to screen the specific antigens. EITB using the specific antigen was established and compared with ELISA. The detection effect of serum and blood on filter paper in EITB was also compared. Results Two specific antigens with isoelectric point (pI) of 9.4 and Mr of 14 000 and 16 600 were obtained, respectively. EITB method based on these antigens was established with the sensitivity and specificity of 92.5% and 100%, respectively, significantly higher than that of ELISA. The sensitivity of serum and filter paper blood was similar in EITB. Conclusion A sensitive and specific EITB method for immunodiagnosis of cysticercosis was established.