皮肤结节性血管炎患者Th1/Th2型细胞因子表达的研究

Expression of Th1/Th2 Cytokines in Patients with Cutaneous Nodular Vasculitis

目的探讨CD3+、CD4+和CD8+T细胞和IL-2、IL-4、IL-6及sIL-2R与结节性血管炎患者发病、疾病活跃程度的关系。方法采用流式细胞仪及ELISA法检测外周血CD3+、CD4+、CD8+T细胞亚群,血清IL-2、IL-6及sIL-2R水平,用链霉亲和素-过氧化物酶(SP)法检测皮损组织局部CD3+、CD4+、CD8+T细胞亚群浸润及IL-2、IL-4表达水平。结果活动期结节性血管炎患者血清IL-2、IL-6及sIL-2R水平高于非活动期和正常人对照组;而外周血CD3+、CD4+、CD8+T细胞亚群与消退期及正常人比较差异无显著性。病变组织中CD3+、CD4+T细胞和IL-2染色阳性细胞高于正常人对照组;IL-4染色阳性细胞与正常人对照组差异无显著性。结论IL-2为代表的Th1细胞因子可能参与结节性血管炎的发病机制,血清及组织浸润IL-2水平与疾病活跃相关。

Objective To investigate the relationship between CD3+T, CD4+T, CD8+T cells, Th1/Th2 cytokines IL-2, IL-4, IL-6, sIL-2R and the pathogenesis as well as disease activity in patients with cutaneous nodular vasculitis (NV). Methods Cytokines IL-2, IL-6, sIL-2R and cell surface markers CD3+, CD4+ and CD8+ were detected by flow cytometry and ELISA with peripheral blood mononuclear cells from patients and controls. Expressions of IL-2, IL-4 and CD3+, CD4+, CD8+ were determined by immunohistochemistry in patients′ lesions in situ. Results Serum levels of IL-2, IL-6 and sIL-2R were significantly increased in patients with active NV compared with those in inactive stage(P < 0.01) and in controls(P < 0.01). There were no significant differences of the percentage of CD3+T, CD4+ and CD8+T in peripheral blood between patients with active NV and patients in stable stage, as well as controls. The numbers of CD3+T, CD4+T cells and IL-2-positive cells, except IL-4 positive cells, were significantly increased in biopsy tissues from NV patients compared with those from normal controls (P < 0.01). Conclusions Th1 immune response, as indicated by the predominance of IL-2, may be involved in the pathogenesis of nodular vasculitis. It is suggested that IL-2 in both sera and tissue infiltration be associated with the disease activity.