脱氧胆酸调节环氧合酶-2对结肠癌细胞增殖的影响

Growth effects of deoxycholic acid on colorectal cancer cell by regulating the expression of cyclooxygenase-2

目的 通过观察不同浓度脱氧胆酸对结肠腺癌细胞SW 1116生长的效应 ,以及在相应状态下细胞内环氧合酶 (COX) 2蛋白表达量的改变 ,探讨脱氧胆酸钠对结肠癌细胞的作用机制。 方法用MTT法测定细胞增殖活性 ;免疫组化及Westernblot方法检测细胞内COX 2蛋白的表达。结果 10～ 10 0 μmol/L的脱氧胆酸钠具有明显的促进结肠腺癌细胞生长的作用 ;大于 10 0 μmol/L时则表现出抑制作用。脱氧胆酸钠在 10、50和 10 0 μmol/L的浓度下均可促进COX 2蛋白的表达 ,10 μmol/L的效应可以持续 72h ,但后两者在 48h后COX 2蛋白表达开始下降。结论 脱氧胆酸对结肠癌细胞SW 1116增殖的影响呈双向调节作用 ,脱氧胆酸促进COX 2蛋白表达可能是其促进结肠癌细胞增殖的作用途径。

Objective The activity and cyclooxygenase (COX-2) protein expression of colorectal adenoc arcinoma cell treated by deoxycholic acids (DCA) were examined in order to find out the carcinogenesis mechanism of DCA. Methods The proliferation of colorectal cancer cells (SW 1116) was detected by MTT metho d . COX-2 protein expression was measured by immunohistochemistry and Western blo t. Results DCA lower than 100 μmol/L concentration can stimulate the growth of the adenoca rcinoma cells with effect reversible, while higher concentration shows the long -acting effect of inhibition. DCA of 10,50 and 100 μmol/L concentration can up -regulate the expression of COX-2 in cells, while only 10 μmol/L can maintain this effect long than 72 hours. The level of COX-2 protein treated by the late r two concentration descends after 48 hours. Conclusion DCA affects the proliferation of SW1116 in two sides. D CA concentration lower than 100 μmol/L can promote COX-2 protein expression, w hich may be the mechanism of its carcinogenesis.