γ射线外照射对胶原代谢等指标的影响

Changes of collagen metabolism induced by ^(60)Co γ-ray external irradiation

目的 通过观察动物模型局部组织胶原代谢指标、以及全身和局部与胶原代谢相关的生化指标 ,以阐明γ射线所致放射性皮肤损伤中胶原代谢变化的分子机理。方法 采用6 0 Coγ射线外照射新西兰兔后臀部皮肤制作皮肤放射损伤模型。实验动物分为正常对照、2 0 ,6 0和 12 0Gy4组 ,14d后处死动物 ,观察局部组织胶原代谢指标及全身和局部与胶原代谢相关的生化指标。结果 (1)γ射线外照射可引起局部组织Ⅲ、Ⅰ型胶原含量下降 ,伴基质金属蛋白酶活性升高 ,组织蛋白分解加强 :(2 )γ射线可引起全血或血浆 5 羟色胺、超氧化物歧化酶、脂质过氧化物量的改变 ,但对它们局部组织含量影响不大。结论 γ射线引起的放射性皮肤损伤机理中胶原代谢变化可能起主导作用。

Objective To study the changes and molecular mechanism of collagen metabolism induced by 60Co γ-ray external irradiation of local tissues. Methods After the hip skin of rabbits was irradiated with 60Co γ-rays,the changes of collagen metabolism and collagen contents of the rabbits' skin of different sex groups were determined. Results The γ-rays decreased the soluble collagen contents (especially type Ⅲ) and increased the activity of MMPs in local tissues.Although the radiation affected the contents of serum SOD,5-HT, and LPO,its influence on the contents in local tissues was not remarkable.There were changes of collagen metabolism in local tissues after radition injury,but no differences were found in SOD,5-HT,LPO,and glycosamine of tissues. Conclusion The main mechanism of skin burn induced by 60Co γ-rays may be that the changes of collagen metabolism bring about the pathological effect.