淋巴细胞DNA双链断裂用于放射损伤检测的可行性研究

Detection of DNA double-strand break of lymphocytes as a biological marker in radiation injury

目的 探讨受照后淋巴细胞DNA双链断裂和凋亡作为放射损伤生物学检测指标的可行性。方法 采用pH值中性彗星电泳方法 ,检测人外周血离体照射 0 1～ 4Gy后 6h和 2 4h淋巴细胞彗星率和彗星尾长 ,并观察其量效关系。结果 照后 6h和 2 4h彗星率呈剂量依赖性上升 ,反应剂量阈值分别为 0 6Gy和 0 1Gy ,r分别为 0 96 7和 0 92 7;照后 6h彗星尾长亦呈剂量依赖性上升 ,r为 0 84 7。结论 中性彗星电泳法结果提示在照射剂量和淋巴细胞彗星率及彗星尾长间存在较紧密的相关性。

Objective To investigate the possibility of applying the DNA double strand break and apoptosis of lymphocytes to biological assay in radiation injury. Methods Comet rates and comet tails were detected using neutral comet assay at 6 h and 24 h following in vitro 0.1-4 Gy γ radiation of whole blood. Results The comet rates increased in a dose-dependent manner.The dose thresholds of comet rates at 6 h and 24 h were 0.6 Gy and 0.1 Gy,respectively.The correlation coefficients were 0.967 and 0.927,respectively.The comet tails at 6 h also increased dose-dependently with a correlation coefficient of 0.847. Conclusion The results of neutral comet assay indicates that there is close correlations between doses and comet rates and tails.