肢体淋巴水肿和透明质酸代谢

Metabolism of hyaluronic acid and extremity lymphedema

目的 探讨淋巴水肿组织中透明质酸的代谢。方法 用埋线法和针刺法收集 39例肢体淋巴水肿皮肤组织液 ,采用放免法检测肢体淋巴水肿透明质酸的含量以及蛋白质的含量。结果 淋巴水肿组织液中透明质酸的含量显著增高 [(2 1× 10 3± 10× 10 3)ng ml直接吸取法 ,(30× 10 3± 4× 10 3)ng ml埋线法 ],是未患病肢体的近 4倍。两组间蛋白质的含量没有明显差异。结论 淋巴循环障碍影响透明质酸的分解代谢 ,使其在皮肤组织中滞留。

Objective To investigate the possible influence of the impairment of lymph fluid on the metabolism of hyaluronic acid(HA) in the lymphedematous skin tissue. Methods Tissue fluid was collected in lymphedematous limbs and the contralateral healthy limbs of 39 patients and HA content was measured with radioimmunoassay.The protein contents were also measured. Results The HA contents in interstitial fluid of lymphedematous limb were significantly (8 fold) higher than that of normal limb.The protein concentration in the tissue fluid did not show significant differences between lymphedema and those with normal tissue. Conclusion The result suggests blockage of regional draining lymphatics may impairs breakdown of HA and the stagnation of HA in the limb may exert a deleterious effect on the interstitium.