体外培养人增生性瘢痕成纤维细胞胶原合成及结缔组织生长因子的表达

Collagen synthesis and expression of connective tissue growth factor in the cultured fibroblasts of human hypertrophic scar

目的 探讨结缔组织生长因子在人增生性瘢痕发病机制中的作用。方法 体外培养人正常皮肤和增生性瘢痕成纤维细胞 ,通过H3 脯氨酸掺入法检测细胞胶原合成 ,通过免疫细胞化学染色和逆转录聚合酶链反应检测细胞结缔组织生长因子蛋白质和mRNA的表达。结果 和正常皮肤成纤维细胞相比 ,增生性瘢痕成纤维细胞的胶原合成和结缔组织生长因子蛋白质及mRNA的表达均显著增高 (P <0 0 1)。结论 结缔组织生长因子可能在增生性瘢痕纤维化过程中发挥重要促进作用。

Objective To explore the effect of connective tissue growth factor on the pathogenesis of human hypertrophic scar. Methods Normal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H 3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods. Results Compared with normal skin fibroblast,the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher( P <0 01). Conclusion Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.