bcl-2和bax基因在喉鳞状细胞癌细胞周期各时相的表达及其意义

The expression and significance of bcl-2 and bax in each phase of the cell cycle in laryngeal carcinoma

目的 检测bcl 2和bax基因在喉癌细胞生长周期各时相的表达情况 ,探讨bcl 2和bax在喉癌细胞周期各时相的表达与喉癌发生、发展及预后的关系。方法 应用流式细胞仪双参数分析结合免疫组化技术和脱氧核苷酸原位末端标记 [terminaldeoxynucleotidyltransferase (TdT) mediateddutp digoxigeninnickend labeling,TUNEL]技术 ,检测 15例声带息肉 (对照组 )和 387例喉鳞状细胞癌 (简称鳞癌 )组织细胞周期各时相的bcl 2、bax表达和细胞凋亡。结果 喉鳞癌组总的bcl 2表达和G0G1期bcl 2的表达明显高于对照组 (P <0 0 5 ) ;而bax的总体表达和在G0G1期、S期及G2M期的表达均明显低于对照组 (P <0 0 5 ) ;喉鳞癌组细胞凋亡明显低于对照组 (P <0 0 5 ) ,而且主要是由于G0G1期细胞凋亡的下降所引起 ;细胞周期各时相的bcl 2、bax表达和细胞凋亡情况与肿瘤的临床分型、临床分期及T分级差异无显著性 ;低分化鳞癌S期、G2M期bcl 2的表达明显高于高分化鳞癌和中分化鳞癌 ;5年内死亡组与复发转移组的S期、G2M期和总的细胞凋亡指数明显增加 (P <0 0 5 ) ,S期和G2M期的bcl 2表达明显增高 (P <0 0 5 )。结论 喉鳞癌细胞中G0G1期bcl 2表达增高 ,G0G1期细胞凋亡的明显下降 ,是喉癌发生的一个重要因素 ;总的bcl

Objective To detect the expression of bcl 2 and bax in each phase of cell cycle in laryngeal carcinoma, and explore the relationship between the expression of bcl 2 and bax in each phase of the cell cycle and the occurrence, development and prognosis in the carcinoma of larynx Methods The immunohistochemical method ,TUNEL technique and flowcytometry(FCM) parameter analyses were combined to detect the apoptosis and the expression of bcl 2 and bax in each phase of the cell cycle in 15 polyps of vocal cord and 387 laryngeal carcinomas Results Total bcl 2 expression and bcl 2 expression in G0G1 stage in laryngeal carcinoma was significantly higher than that in polyp of vocal cord In contrast, the total bax expression and the bax expression in each phase of cell cycle in laryngeal carcinoma were all lower than that in polyp of vocal cord The total apoptosis index in laryngeal carcinoma was obviously lower than that in polyp of vocal cord, and this phenomenon was mainly caused by the decrease of the apoptosis in G0G1 phase The bcl 2, bax expression and the apoptosis wasn′t notably related to clinical stage, clinical type and T grade In poor differentiated squamous carcinoma, the bcl 2 expression in S and G2M phase was obviously higher than that in well differentiated and the moderate differentiated squamous carcinoma The total apoptosis index, the apoptosis in S phase and the apoptosis in G2M phase were obviously enhanced both in the group of recurrence and in the patients who died in 5 years after the operation, in the same samples, the significant increasing of bcl 2 expression in S and G2M phase was detected Conclusions The significant decreasing of the apoptosis in G0G1 phase caused by high expression of bcl 2 was an important affair in the initial stage of laryngeal carcinoma Accompanying the significant increasing of the total apoptosis index, the apoptosis in S phase and the apoptosis in G2M phase could be regard as an indicator that the cancer of larynx was malignant with poor prognosis and need adjuvant therapy The decreasing of bax expression may play a role in the occurrence of laryngeal carcinoma