8-溴-环磷酸腺苷和异戊烯焦磷酸不改变神经细胞系tau蛋白的表达

Effect of 8-Br-cAMP and IPP on the expression of tau protein in various neural cell lines

目的 分析 8 溴 环磷酸腺苷 (8 Br cAMP)和异戊烯焦磷酸 (IPP)在信号传导水平上对不同神经细胞系tau蛋白表达的影响。 方法 不同的神经细胞先用 8 Br cAMP和IPP处理 3 0min ,然后细胞内的tau蛋白被富集 ,tau蛋白的表达用蛋白印迹方法来检测。 结果 SH SY 5Y细胞仅表达分子量最小的tau蛋白 ;PC12细胞主要表达高分子量tau蛋白 ,但也表达少量异构体型tau蛋白 ;NG10 8 15细胞主要表达tau蛋白 ,含量较少 ,呈现 3种异构体 ,但也表达少量高分子量tau蛋白。在本实验条件下 ,未发现 8 Br cAMP和IPP改变这些细胞tau蛋白的表达。 结论 8 Br

ObjectiveTo study the effect of 8-Br-cAMP and isopentenyl pyrophosphate (IPP) on the ex pression tau protein in various neural cell lines at the level of signal transdu ction. MethodsNG108-15, PC12 and SH-SY5Y cells were treated with 8-Br-cAMP and IPP in the 37°C incubator for 30 min, then the tau-enriched samples were analyzed by West ern blot. ResultsSH-SY5Y cells only expressed the isoform of tau protein with the lowest molecul ar weight; PC12 cells mainly expressed high molecular weight tau, and several ot her isoforms of tau; NG108-15 expressed a little of isoform of tau. In the expe rimental conditions, the expression of tau was altered with 8-Br-cAMP and IPP in NG108-15, SH-SY5Y and PC12 cells. Conclusions8-Br-cAMP and IPP does not alter the expression of tau in NG108-15, SH-SY5Y and PC12 cells.