哮喘患儿单个核细胞表面CD86分子表达和T_H源细胞因子水平及之间相关性研究

Expression of CD86 on monocyte and B cell surface,the level of T_H-derived cytokine and their correlation in children with asthma

目的 探讨哮喘急性发作患儿T细胞协同刺激途径和T细胞亚群激活状况。方法 随机选择哮喘急性发作期患儿 35例 ,对照组 31例。用直接免疫荧光流式细胞术检测外周血单个核细胞 (PBMC)中白细胞分化抗原 14阳性 (CD+ 14 )细胞表达CD86的平均荧光强度 ;经脂多糖刺激后PBMC中CD+ 19细胞百分率及CD+ 19CD+ 86双阳性细胞百分率 ;ELISA检测植物血凝素刺激后PBMC培养上清白细胞介素 (IL) 4、IL 13、IFN γ水平及血浆总IgE水平 ,并分析它们之间的相关性。 结果 (1)哮喘组CD+ 14 细胞表达CD86的平均荧光强度 (31 8± 9 2 )、CD+ 19细胞百分率 [(13 5± 4 0 ) % ]、CD+ 19CD+ 86细胞百分率 [(4 6± 2 0 ) % ]及血浆总IgE水平 [186 6 (6 4 3～ 72 3 6 )kIU/L]与对照组 [(2 3 5±6 4 )、(8 2± 3 0 ) %、(2 3± 1 4 ) %、4 2 0 (0 9～ 115 2 ) ]比较差异有显著性 ;(2 )哮喘组IL 4和IL 13水平均增高 ,IFN γ水平降低 ,与对照组比较差异有显著性 ;(3)哮喘组CD+ 14 细胞的CD86平均荧光强度与CD+ 19CD+ 86细胞百分率、CD+ 19CD+ 86百分率与血浆总IgE水平及CD+ 19、CD+ 19CD+ 86百分率与IL 4、IL 13水平均呈显著正相关。结论 哮喘急性发作患儿PBMC中抗原递呈细胞表达CD86的强度或数量?

Objective To explore the role of T lymphocytes activation co-stimulation pathway and T lymphocyte subset activation in asthma pathogenesis.Methods The blood samples were taken from 35 asthma children (including 22 male and 13 female, age 11 months-9 years) and 31 normal children (including 19 male and 12 female, age 8 months-12 years). Direct immunofluorescence flow cytometry was used to detect the CD86 mean fluorescence intensity (MFI) on CD +_ 14 cell, CD +_ 19 cell percentage and CD19 and CD86 double positive cell percentage in PBMC activated by LPS. ELISA was used to detect the levels of IL-4, IL-13, IFN-γ in culture supernatants of PBMC stimulated with PHA and plasma total IgE level. Results (1) The CD86 MFI on CD +_ 14 cell in asthma group was elevated significantly (31.8±9.2 vs 23.5±6.4, P <0.01). (2) CD +_ 19 cell percentage and CD19 and CD86 double positive cell percentage in PBMC stimulated with LPS in asthma group were increased significantly(13.5±4.0 and 4.6±2.0 vs. 8.2±3.0 and 2.3±1.4, respectively, P <0.01). The plasma total IgE level [186.6(64.3-723.6)] was higher than that of control′s [ 41.95(0.9-115.2)].(3)The levels of IL-4 and IL-13 in supernatants stimulated with PHA increased significantly [34.2(2.8-70.0) and 1 239.0 (378.3-2 929.0) vs. 9.7(2.0-46.2) and 683.2(128.8-1 560.8) respectively, P <0.01, P <0.05];and the level of IFN-γ decreased significantly [12.16(1.6-44.8) vs. 26.7(2.1-99.5) P <0.05]. (4) In asthma group there was a significantly positive correlation of CD86 MFI on CD +_ 14 cell with CD19 and CD86 double positive cell percentage ( r =0.644), there was a significantly positive correlation of CD +_ 19 CD +_ 86 cells percentage with plasma total IgE ( r =0.537);there was a significantly positive correlation of CD +_ 19 cells percentage with the levels of IL-4 and IL-13 ( r =0.607, 0.540 respectively);a significantly positive correlation of CD +_ 19 CD +_ 86 percentage cells with the levels of IL-4 and IL-13 was found ( r =0.617, 0.678, respectively). Conclusions(1)The expression of CD86 on APC cells increased in children with acute asthma. (2) The response to mitogens of B lymphocyte and T_H2 but not T_H1 lymphocyte were increased significantly. The results suggest that CD86 and imbalance of T_H subset may play an important role in asthma pathogenesis.