以聚β-羟基丁酸酯为载体的肾上腺细胞移植实验研究

Transplantation of adrenocortical cells among allorat using ploy-β-hybroxybutyric acid as cell carrier

目的 探讨以聚 β 羟基丁酸酯 (PHB)作为细胞载体进行肾上腺细胞移植的效果。 方法 Wistar大鼠切除双侧肾上腺后 ,分别行单纯肾上腺细胞移植和以PHB为载体的肾上腺细胞移植 ,术后动态观察受者的血清皮质酮和醛固酮水平以及移植物组织学形态。结果 肾上腺细胞移植后 ,大鼠血清皮质酮和醛固酮的水平升高 ,以PHB为载体的细胞移植组升高更加显著 ,形态学证实PHB载体组的肾上腺细胞生长良好。结论 以PHB为载体的肾上腺细胞移植比单纯肾上腺细胞移植效果更好。

Objective To investigate the possibility of transplantation of adrenocortical cells to treat insufficiency of adrenocortical function using poly-β-hybroxybutyric acid (PHB) as cell carrier.Methods Adrenocortical cells were seeded to PHB and then implanted into rats bearing bilateral adrenalectomy, while adenocortical cells implanted into the similar rats as a contrast. The changes of blood corticosterone and aldostenone and local histological changes in these rats were observed.Results The blood corticosterone and aldostenone were elevated after transplantation. The levels of blood corticosterone and aldostenone in PHB group were higher than that of control group. Histological study showed that adenocortical cells in PHB grew well.Conclusion The implanted adrenocortical cells within PHB can not only survive in rat body but also secrete corticostenone and aldostenrone which can satisfy the need of adrenalectomized rats. It is more effective to use PHB in adrenocortical cell transplantation than that cell implanted singly.