多重聚合酶链反应快速诊断感染性眼内炎的研究

Infected endophthalmitis diagnosed by multiplex polymerase chain reaction

目的 探讨多重聚合酶链反应 (MPCR)快速诊断细菌性、真菌性眼内炎及混合感染的价值。 方法 MPCR法检测常见致病性细菌和真菌菌株 ,并检测 38例患者 4 1只眼 (双眼 3例 ,单眼 35例 )眼内液标本 ,与细菌和真菌培养结果比较。 结果 MPCR法 5 h即可检测出标本中微量细菌和真菌 ,4 1份标本 MPCR检测阳性 34份 (82 .9%) ,其中细菌阳性 2 6份 ,真菌阳性 6份 ,细菌和真菌均为阳性 2份 ,MPCR阳性率明显高于细菌或真菌培养 (χ2 =9.6 0 ,P<0 .0 5 )。 结论 MPCR速度快、敏感性和特异性高 ,有助于细菌性和真菌性眼内炎的快速明确诊断。

Objective To evaluate the rapid diagnosis of bacterial and (or) fungal endophthalmitis by multiplex polymerase chain reaction (MPCR). Methods MPCR was performed to detect the DNA segment of bacteria and (or) fungi from standard strains and 41 samples of intraocular fluid or vitreous from 38 patients (3 with double eyes and 35 with single), and the results were compared with the cultured bacteria and fungi. Results Five hours after detected by MPCR, bacteria and (or) fungi in 34 out of 41 samples (82.9%) from patients were detected,including bacteria in 26,fungi in 6,and both bacteria and fungi in 2. The positive rate of MPCR was obviously higher than the cultured ones( χ 2 =9.60, P< 0.05). Conclusion With the advantages of rapidity, sensibility, and specificity, MPCR can make for the rapid and definitive diagnosis of bacterial and (or) fungal endophthalmitis.