兔眼脉络膜黑色素瘤的第二代光敏剂光动力治疗

Photodynamic therapy of melanomas in rabbit's eyes using chloroaluminum sulfonated phthalocyanine

目的 观察第二代光敏剂 CASPc(chloroaluminum sulfonated phthalocyanine,CASPc)对B16 F10黑色素瘤动物模型的光动力治疗 (PDT)作用。 方法 38只新英格兰大白兔经免疫抑制后种植B16 F10黑色素瘤碎片于脉络膜下腔 ,B型超声、间接检眼镜随访肿瘤生长至 2 .0～ 3.8mm厚时 30只兔眼给予 PDT治疗。耳静脉注射 CASPc 5 mg/kg,2 4 h后给予氩 -染料激光照射 ,激光波长 6 75 nm,输出功率6 0 0 nm/cm2 ,激光照射剂量 2 0～ 70 J/cm2 ;8只对照组兔眼只给予激光或光敏物质。治疗后观察 6～ 8周。 结果 38只兔眼建模成功。 30只 PDT治疗兔眼中 2 1只眼肿瘤得到控制 ,9只眼未能控制 ;对照组兔眼肿瘤治疗后 2周充满整个玻璃体。 结论 第二代光敏剂 CASPc对实验性眼 B16 F10黑色素瘤具有一定的PDT治疗作用。

Objective To evaluate the effectivness of photodynamic therapy (PDT) with chloroaluminum sulfonated phthalocyanine (CASPc) in B16F10 melanomas in a rabbit model. Methods B16F10 murine melanoma tumor fragments were implanted transclerally into the subchoroidal space of 38 immunosuppressed New Zealand albino rabbits and examined with indirect ophthalmoscopy and ultrasonography. When the tumors ranged from 2.0~3.8 mm in height, 30 rabbits were treated by PDT, with intravenous injection of CASPc 5 mg/kg, and irradiation at the wavelength of 675 nm of an argon-pumped dye laser after 24 hours. Light dose ranged from 20 J/cm 2 to 70 J/cm 2. The other 8 animals were treated with light only or photosensitizer only. The animals were followed up for 6~8 weeks. Results The 30 tumor-bearing rabbits were by PDT (laser and CASPc) regressed in 21 animals after treatment. At light doses under 40 J/cm 2, tumor regrowth was observed in 9 animals after two weeks of treatment. In all of the 8 control animals, the tumor-bearing eyes were filled with tumor at the third week after implantation with laser doses of 70 J/cm 2. Conclusion The study suggest that CASPc PDT may be effective in the treatment of B16F10 choroidal melanomas.