中国北方汉族IgA肾病患者UG基因G38A基因多态性与临床和预后的关系

Uteroglobin G38A polymorphism is associated with the progression of IgA nephropathy in Chinese patients

目的 探讨中国北方汉族IgA肾病患者UG基因G3 8A基因多态性的分布及与临床和预后的关系。方法 选 3 0 0例北方汉族IgA肾病患者 (对其中 93例患者进行 2～ 6年的随访。)及北方汉族健康献血者 14 5例 ,盐析法提取外周血基因组DNA ,PCR 限制性片段长度多态性法确定UG基因G3 8A基因型 ,分析不同基因型与IgA肾病临床、病理及预后的关系。结果 (1)IgA肾病患者UG基因的 3种基因型 (3 8AA、3 8AG和 3 8GG)与健康对照者间差异无显著性 (P >0 0 5)。 (2 ) 3种基因型与IgA肾病患者的性别、肾穿刺时的年龄、血尿、蛋白尿、肾功能、血清IgA水平及病理间无显著关系。 (3 )相对于其他基因型 ,3 8AA型预后差 (OR =2 3 7,95%CI =1 12～ 5 0 1,χ2 =8 2 48,P <0 0 1)。结论 UG基因G3 8A基因多态性与中国北方汉族人群IgA肾病的发病及临床无关 ,3 8AA基因型可能是IgA肾病慢性化进展的危险因子之一。

ObjectiveUteroglobin is a multifunctional protein Both uteroglobin gene knockout and antisense transgenic mouse models developed the pathological and clinical features of IgA nephropathy Uteroglobin G38A polymorphism has been reported to be associated with the progression of IgA nephropathy in some Caucasian and Asian populations, but there are no documents on Chinese population The present report was to investigate the associations of uteroglobin G38A polymorphism with the development and progression of IgA nephropathy in Chinese patients MethodsThree hundred patients with biopsy proven IgA nephropathy were identified from Renal Disease database Ninety-three patients had been followed-up for 2-6 years 145 healthy donors served as normal controls Genomic DNAs were extracted from peripheral blood leucocytes The uteroglobin G38A polymorphism was determined by PCR-RFLP Uteroglobin G38A genotype and allele frequency were compared between patients with IgA nephropathy and normal controls In addition, associations of G38A polymorphism with blood pressure, hematuria, proteinuria, pathological lesions and prognosis of renal function were analyzed in patients with IgA nephropathy ResultsDistribution of uteroglobin G38A polymorphism in patients with IgA nephropathy (38AA:13 2%; 38AG:57 6%;38GG:30 2%) and normal controls (38AA:18 2%;38AG:60%; 38GG:21 8%;P>0 05) showed no difference But patients with the 38AA genotype showed a higher odds ratio for progression of renal function (OR=2 37, 95%CI=1 12-5 01) as compared to patients with the 38AG/GG genotype ConclusionUteroglobin G38A polymorphism had no association with the development of IgA nephropathy, but the homogenous 38AA genotype maybe one of the genetic markers for disease progression in Chinese IgA nephropathy