血管紧张素Ⅱ1型受体在肝纤维化形成过程中表达变化的研究

Change of expression of angiotensin Ⅱ type 1 receptor in the formation of liver fibrosis

目的 探讨血管紧张素Ⅱ 1型受体 (angiotensinⅡtype 1receptor,AT1R)在不同程度纤维化肝脏组织中的表达情况。方法 采用免疫组化法检测Ⅰ型胶原 ;采用间接免疫荧光标记法进行AT1R检测 ,同时应用半定量逆转录聚合酶链反应 (RT PCR)法检测AT1RmRNA的表达。结果 Ⅰ型胶原面积随肝纤维化程度增加而增加。AT1R阳性表达主要分布在肝小叶周边及肝窦区星形细胞(HSC)的胞质内。 12例正常肝组织中 8例呈阳性表达 ,18例纤维化肝组织均呈阳性表达 ,纤维化肝脏组AT1R阳性表达细胞数明显多于正常肝脏组 (P <0 .0 0 1) ,并随Ⅰ型胶原面积的增加而明显增加。纤维化肝脏组AT1RmRNA的相对表达量明显高于正常肝脏组 (P <0 .0 1)。结论 随着肝组织纤维化程度的增加 ,AT1R及AT1RmRNA的表达量均明显增多 ,AT1R在肝纤维化发生发展过程中可能具有重要的作用。

Objective To detect the expression of angiotensin Ⅱ type 1 receptor (AT1R) in the different fibrotic stages of human liver. Methods The collagen Ⅰ was detected by immunohistochemical method. Indirect immunofluoresence-labelled method was used to detected AT1R. And semi-quantitative RT-PCR was used to detect AT1R mRNA. Results The type Ⅰ collagen area enlarged with the progression of hepatic fibrosis. The positive expression of AT1R was concentrated in the cytoplasm of hepatic stellate cells(HSC) around the lobes of liver or in the hepatic sinus. Eight of twelve cases of normal liver tissues were positive, and all the eighteen cases of fibrotic liver tissues were positive. The positive cell number in fibrotic liver tissues was significantly higher than that in normal ones (P<0.001), and the positive cell number increased as collagen Ⅰ area enlarged. Conclusions The fact that expressions of AT1R protein and AT1R mRNA both enhance as hepatic fibrosis intensifies indicates that AT1R may play an important role in the development of hepatic fibrosis.